| Transzgén/Vonal | Jax azonosító | Pubmed azonosító | Kontakt személy | Vonal eredeti tulajdonosa |
| BAC_pva/gfp 2 | | 12177202 | Szabó Gábor | Hannah Monyer* |
Pva-gfp transgenic mice express gfp in parvalbumin-expressing neurons and muscle.
* BAC construct was designed by AH Meyer in Monyer's lab. Pronuclear microinjection was carried out in IEM-HAS. |
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| camK2A/gfp 22 | | | Szabó Gábor | Szabó Gábor |
CamK2A-gfp transgenic mice express gfp in piramidal and purkinje cells.
Construct and microinjection were carried out in IEM-HAS. |
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| D2 (f) Tm | - | 23524969 | Gereben Balázs | AC Bianco, Miami FL, Gereben B. |
| floxolt kettes-típusú dejodáz enzim |
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| D3 (f) Tm | - | - | Gereben Balázs | D. Salvatore, Nápoly |
| floxolt hármas-típusú dejodáz enzim |
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| gad65_3e/gfp 5.5 30 | | 15115742 | Szabó Gábor | Szabó Gábor |
| gad65-gfp transgenic mice express gfp in interneurons under the control of gad2 promoter and 5.3 kb length upstream region. |
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| gluRa2 (f) Tm | | 17296559 | Hájos Norbert | Hannah Monyer |
| floxed-GluR1 mouse strain, Fuchs et al., 2007 Neuron |
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| gluRd2 (f) Tm | | 17296559 | Hájos Norbert | Hannah Monyer |
| floxed-GluR4 mouse strain, Fuchs et al., 2007 |
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| Gt(ROSA)26Sor_CAG/tdTomato Tm | 007905 | 20023653 | Erdélyi Ferenc | Hongkui Zeng, Allen Institute for Brain Science (AIBS) |
| These Ai9 mice harbor a targeted mutation of the Gt(ROSA)26Sor locus with a loxP-flanked STOP cassette preventing transcription of a CAG promoter-driven red fluorescent protein variant (tdTomato), and may be useful as a Cre reporter strain. TdTomato is expressed following Cre-mediated recombination. |
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| Gt(ROSA)26Sor_CAG/ZsGreen1 Tm | 007906 | 20023653 | Erdélyi Ferenc | Hongkui Zeng, Allen Institute for Brain Science (AIBS) |
| These Ai6 mice harbor a targeted mutation of the Gt(ROSA)26Sor locus with a loxP-flanked STOP cassette preventing transcription of a CAG promoter-driven enhanced green fluorescent protein (ZsGreen1), and may be useful as a Cre reporter strain. ZsGreen1 is expressed following Cre-mediated recombination. |
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| Gt(ROSA)26Sor/CAG/GCaMP3 Tm | 014538 | 22378886 | Erdélyi Ferenc | Hongkui Zeng, Allen Institute for Brain Science (AIBS) |
| Ai38 mice express the fluorescent calcium indicator protein GCaMP3 after exposure to Cre recombinase. Following subsequent calcium binding (such as neuronal activation), bright EGFP fluorescence is observed. |
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| GT(ROSA)26Sor/Cre - | | 18799753 | Erdélyi Ferenc | |
| The Cre Deleter mouse was developed by Artemis Pharmaceuticals (now Taconic). The mutant was created by a targeted mutation knock-in of Cre into the Gt(ROSA)26 locus. Cre is expressed under the control of the Gt(ROSA)26Sor gene. |
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| nestin/cre_ERT2 Tg | | 18160632 | Fekete Csaba | G Fishell, New York, NY |
| Tamoxifen-indukálható Cre rekombináz nestin promóter alatt |
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| pva/IRES_cre - | 017320 | 17190606 | Szabó Gábor | Silvia Arber |
| PV-Cre knockin mice express Cre recombinase in parvalbumin-expressing neurons (such as interneurons in the brain and proprioceptive afferent sensory neurons in the dorsal root ganglia), without disrupting endogenous Pvalb expression. |
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| rax/cre-ert2 Tg | | | Fekete Csaba | Seth Blackshaw |
| Az állat a Rax promoter alatt tamoxifen indukálható Cre-t termel. Felnõtt állatban az expresszió tanicita specifikus. |
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| sb/cag/yfp/sb 27 | | | Erdélyi Ferenc | Szabó Gábor |
| Sleeping Beauty-CAG-Venus construct was made by Lajos Mátes (BRC). Transgene is integrated in one copy. Fluorescent protein expression can be found in endothelial cells and in Purkinje cells in brain. |
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| Tau/LSL/mgfp_iresNlacZ Tm | B6;129P2-Mapttm2Arbr/J | 15836427 | Fekete Csaba | Silvia Arber |
Description
In these TaumGFP mice, Cre recombinase activity induces expression of membrane bound green fluorescent protein (mGFP) and nuclear targeted beta-galactosidase in neurons. mGFP, MARCKS ("myristoylated alanine-rich C-kinase substrate") protein fused to green fluorescent protein, targets GFP expression to the membrane, and IRES-NLS-lacZ targets expression of beta-galactosidase to the nucleus. A floxed STOP cassette prevents expression of the reporter genes until removed by Cre recombinase activity. The construct replaces the endogenous start codon in exon 2. Homozygotes are viable and fertile.
Development
A targeting vector designed by Drs. Silvia Arber and Markus Sigrist (Biozentrum, University of Basel) containing floxed STOP sequence, membrane-targeted green fluorescent protein, nuclear targeted beta-galactosidase and polyadenylation signal sequence (loxP-STOP-loxP-mGFP-IRES-NLS-lacZ-pA) was inserted into exon 2. The construct was electroporated into 129P2/OlaHsd derived E14 embryonic stem (ES) cells. Correctly targeted ES cells were injected into blastocysts. The resulting chimeric animals were tested for germline transmission. The mice were crossed to C57BL/6JRccHsd mice. Upon arrival at The Jackson Laboratory, the mice were crossed to C57BL/6J (Stock No. 000664) at least once to establish the colony. |
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